The Knowledge Graph Report
Nobel to Product / Issue 01
Scientific Citations × Patent Filings
A study in citation cascade

Nobody read it for fifteen years.
Then the world needed a vaccine,
and billions of doses cited it.

How a 2005 paper by Katalin Karikó and Drew Weissman — a quiet finding about modified nucleosides in RNA — became the intellectual spine of the Moderna, Pfizer–BioNTech, and CureVac vaccines. A story only visible when you overlay scientific citations with patent filings.

The lede

In August 2005, the journal Immunity published a paper that nobody outside a small corner of RNA biology particularly wanted to read. Its title was forbidding: "Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA." The finding was subtle: if you substitute the uridine in synthetic mRNA with pseudouridine, the immune system stops treating it as foreign. You can inject it. It can make protein. It will not trigger an inflammatory storm.

For fifteen years, that finding sat in the scientific literature, accruing citations at the quiet pace of a successful but specialised paper. Katalin Karikó was demoted at Penn. Then, in January 2020, a novel coronavirus was sequenced, and two small companies — Moderna and BioNTech — reached for the only chemistry that would let them turn a genetic sequence into a vaccine in weeks instead of years. Both had already licensed the Karikó–Weissman patents. Both used 1-methyl-pseudouridine. The 2005 paper, once ignored, became the most consequential piece of chemistry of the twenty-first century.

This dashboard shows what that cascade looks like, scientifically and commercially, in the same view. Every circle on the left is a paper; every square on the right is a patent. The story emerges only when you see both at once.

Citations to the 2005 paper
2,437
citing publications (Scite)
Smart Citations
2,125
44 supporting · 4 contrasting · 2,039 mentioning
Doses administered globally
13.6B
mRNA COVID-19 vaccines, 2020–2024
Nobel Prize Awarded
2023
Karikó & Weissman, Physiology or Medicine
§ 01 / The citation explosion

For fifteen years, almost nothing.

Then, in 2020, the world discovers the paper all at once. Annual citations to Karikó et al. 2005, by year.

How to read this. Each bar is one year's citations to the 2005 paper. The dormant period before 2020 is not an artifact — it reflects a genuinely small specialist literature. The 2020–2022 spike is what happens when a basic-science result becomes the enabling chemistry of a global public-health response. Distribution estimated from Scite's 2,437 total citing publications.

§ 02 / Bench to billions

One graph. Papers and patents.

The journey from foundational science to intellectual property to clinical evidence to Nobel Prize — rendered as a single timeline with two columns.

Scientific paper
Patent family
Foundational paper (2005)
◀ Scientific Papers
Year
Patents ▶
2005
Immunity · Aug 2005 · The foundational paper
Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA
Karikó, Buckstein, Ni, Weissman
2,437 citing publicationsDOI 10.1016/j.immuni.2005.06.008
2008
Molecular Therapy · Penn
Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability
Karikó, Muramatsu, Welsh et al.
1,656 citations
2010
Nucleic Acids Research · Penn
Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation
Anderson, Karikó et al.
570 citations
2011
Nucleic Acids Research · Penn
Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation
Karikó, Muramatsu, Ludwig et al.
895 citations
2012
Molecular Therapy · in vivo proof
Increased erythropoiesis in mice injected with submicrogram quantities of pseudouridine-containing mRNA encoding erythropoietin
Karikó, Muramatsu, Keller et al.
276 citations
ModernaTX · Filed 2012
Modified Nucleoside, Nucleotide, And Nucleic Acid Compositions
EP 2791160 B1 · 49 patents in family
352 forward citationsModerna
2013
ModernaTX · Filed 2013
Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US 11,564,998 B2 · 146 patents in family
525 forward citationsModerna
2014
Nat. Rev. Drug Discovery · with BioNTech co-authors
mRNA-based therapeutics — developing a new class of drugs
Karikó, Şahin, Türeci
2,251 citations
2015
J. Controlled Release · LNP delivery
Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice
Pardi, Tuyishime, Karikó et al.
865 citations
2016
Acuitas Therapeutics · Filed 2016
Lipids and lipid nanoparticle formulations for delivery of nucleic acids
US 11,040,112 B2 · The LNP chemistry that ships in BNT162b2
241 forward citationsAcuitas
2017
ModernaTX · Filed 2017
High Purity RNA Compositions And Methods For Preparation Thereof
US 11,202,793 B2 · Manufacturing backbone
57 forward citationsModerna
2018
ModernaTX · Filed 2018
Nucleic acid vaccines
US 10,709,779 B2 · The platform patent for mRNA-1273
163 forward citationsModerna
2020
NEJM · July 2020 · Phase 1
An mRNA Vaccine against SARS-CoV-2 — Preliminary Report (mRNA-1273)
Jackson, Anderson, Rouphael et al.
3,460 citations
NEJM · Dec 2020 · The trial that changed everything
Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine
Polack, Thomas, Kitchin et al.
15,829 citations
BioNTech SE · Filed 2020
Coronavirus vaccine (BNT162b2 platform)
US 12,133,899 B2 · 44 patents in family · Şahin, Türeci, Muik et al.
17 forward citationsBioNTech
2021
NEJM · Feb 2021 · Phase 3 efficacy
Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine
Baden, El Sahly, Essink et al.
10,598 citations
2022
Immunity · review of the platform
Innate immune mechanisms of mRNA vaccines
Verbeke, Hogan, Loré, Pardi
314 citations
Science · the platform expands
A multivalent nucleoside-modified mRNA vaccine against all known influenza virus subtypes
Arevalo, Bolton, Le Sage et al.
219 citations
2022
U. Penn · Granted 2023 (filed 2022) · The Karikó–Weissman patent
RNA Containing Modified Nucleosides and Methods of Use Thereof
US 11,801,314 B2 · 27 patents in family · Karikó & Weissman
336 forward citationsFoundational IP
2023
★ Nobel Prize in Physiology or Medicine
Awarded to Karikó & Weissman "for their discoveries concerning nucleoside base modifications that enabled the development of effective mRNA vaccines against COVID-19"
Eighteen years after the 2005 paper
2024
U. Penn · Granted 2024 · Karikó & Weissman
Purification and Purity Assessment of RNA Molecules Synthesized with Modified Nucleosides
US 12,054,734 B2 · 5 patents in family
79 forward citationsPenn / Karikó–Weissman
§ 03 / The patent landscape

The commercial architecture.

Ten patents, four assignees, one chemistry. Every mRNA COVID-19 vaccine shipped under the permission structure below — and every one cites the Karikó–Weissman line of work.

§ 04 / Follow the science

What the later work actually says.

Smart Citations — the exact sentences in which later papers and patents credit the 2005 breakthrough. This is citation not as a number but as narrative.

Citing paperVerbeke, Hogan, Loré & PardiInnate immune mechanisms of mRNA vaccines2022
"Decades of basic research were needed to understand how to circumvent the problems posed by innate immune recognition of mRNA. A major breakthrough came via the studies of Karikó, Weissman and colleagues in the 2000s, who discovered that the presence of certain modified vs. unmodified ribonucleosides works as a molecular mechanism by which cellular RNA sensors discriminate self RNA from foreign RNA."
Immunity, vol. 55, p. 1993 · Section: Overview of nucleoside-modified mRNA and iLNP technologies
Citing paperPolack, Thomas, Kitchin et al.Safety and Efficacy of BNT162b22020
"BNT162b2 is a lipid nanoparticle–formulated, nucleoside-modified RNA (modRNA) encoding the SARS-CoV-2 full-length spike, modified by two proline mutations to lock it in the prefusion conformation."
New England Journal of Medicine · Introduction · The first sentence of the mRNA vaccine era in clinical practice
Citing paperKarikó, Muramatsu, Keller et al.Increased Erythropoiesis from ψ-mRNA2012
"When modified nucleosides, such as pseudouridine, were incorporated into the RNA, it no longer activated TLR7, TLR8, RIG-I, PKR, and 2′-5′-oligoadenylate synthetase. More importantly, pseudouridine-containing mRNA was not only translatable, but higher amounts of protein were produced compared to uridine-containing mRNA."
Molecular Therapy, vol. 20 · Introduction · The eight-year-on confirmation, by Karikó herself, of why 2005 mattered
Citing patentKarikó & Şahin (BioNTech SE)Method for reducing immunogenicity of RNA2020
"Pseudouridine-modified mRNA was found to be highly translatable and non-immunogenic (see Karikó, K., Muramatsu, H., Welsh, F. A., Ludwig, J., Kato, H., Akira, S., and Weissman, D. (2008) Incorporation of pseudouridine into mRNA…)"
US 11,873,478 B2 · Background section · Karikó herself, now a BioNTech co-inventor, citing her Penn-era work inside the commercial IP of the vaccine
The reason this view is unique
No other database can draw this picture in a single frame. A citation database will show you the papers. A patent database will show you the IP. Neither, alone, tells the story of what actually happened between 2005 and 2023 — which is that a single specialist finding in RNA biochemistry migrated from the scientific literature into a commercial patent estate, was licensed to Moderna and BioNTech, became the chemistry of BNT162b2 and mRNA-1273, generated 13.6 billion doses of vaccine, saved an estimated 20 million lives in its first year of deployment, and won its authors the Nobel Prize — all while accumulating the citation curve you can see in § 01.

The graph above is the story. You need both halves to see it.